Experimental and theoretical analyses of the lactate dehydrogenase andglutathione reductase based enzymatic AND logic gates in which the enzymes andtheir substrates serve as logic inputs are performed. These two systems areexamples of the novel, previously unexplored, class of biochemical logic gatesthat illustrate potential biomedical applications of biochemical logic. Theyare characterized by input concentrations at logic 0 and 1 states correspondingto normal and abnormal physiological conditions. Our analysis shows that thelogic gates under investigation have similar noise characteristics. Bothsignificantly amplify random noise present in inputs, however we establish thatfor realistic widths of the input noise distributions, it is still possible todifferentiate between the logic 0 and 1 states of the output. This indicatesthat reliable detection of abnormal biomedical conditions is indeed possiblewith such enzyme-logic systems.
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